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April 2024
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Organic Pumpkin Protein Powder

The Organic Pumpkin Protein Powder that we provide meets all the
nutraceutical benefits of a vegetable protein along with the advantages
of a product of the highest quality and purity.

USDA Organic Certified

Organic Certified




Wild Harvested

Origin and history

Cucurbita pepo L. (Pumpkin) is a plant native to Mexico and the USA belonging to the Cucurbitaceae family. It has been domesticated in the New World for thousands of years. This familyincludes cucumbers, melons, watermelon, loofahs, pumpkins, squashes, and gourds. Several species of Cucurbitaceae have been widely used in traditional medicine in Central and North America as a diuretic and as a de-worming treatment. In Ayurvedic medicine, Cucurbita pepo seeds are considered as a diuretic, a cure for bronchitis, fever and effective against parasitic worms.1,2


The pumpkin seeds, known as an important nutritional source are oval, flattened, smooth and light to dark beige color. The seeds are usually cooked, toasted or ground for types different of consumption, as an appetizer, eaten whole. Also, they are ingredient of salads, sauces, cakes, breads additives. Pumpkin seeds have a high protein content and are an important source of minerals such as iron and potassium.3


45 %

Specification: 45% Protein

Botanical Name: Cucurbita pepo L.

Ref Number: TGLPPP

Tested by: QC

Punkin Seeds Chemical Composition


Pumpkin seeds contain large quantities of naturally occurring proteins around 35% and unsaturated fatty acids (mainly linoleic acid and oleic acid), tocopherols, and phytosterols. Pumpkin seeds are a source of zinc, phytochemicals as stigmasterol, β-sitosterol, the anthelmintic cucurbitin, squalene, tocopherols, cardio-protective fatty acids which can prevent chronic diseases.4


Flaxseed is known as the richest dietary source of lignans; however, pumpkin seeds contain secoisolariciresinol, a phytoestrogens of the lignan type. Phytoestrogens of the lignan type are widely distributed in plant-derived food and they have potential health benefits.5


Other compound found in sarcocarp and pumpkin seeds is Cucurmosin. This phytochemical is considered toxic to cancer cells and protects against viral, fungal, and bacterial agents. Curcumosin has been shown to induce apoptosis in pancreatic cancer and myeloid leukemia cells.6-8


Nutritional composition and nutraceutical properties of Pumpkin Seed Protein Powder 45%.


Pumpkin Seed Protein Powder 45% is an ingredient rich in protein, obtained from pumpkin seeds. Pumpkin Seed Protein Powder 45% is considered a Super Food due to its balanced nutrient content with pumpkin protein 45% (amino acids including tryptophan), sterols plant, fiber, vitamins K, C, B3, B6, beta carotene, pantothenic acid folate, and minerals as iron, magnesium, zinc, calcium, potassium, phosphorus, calcium. Moreover, pumpkin seed powder is low in fats and carbohydrates. The nutraceutical properties of pumpkin seeds have been investigated in recent years finding many health benefits.


Effects on the Central Nervous System (CNS).


Pumpkin Seed Protein Powder 45% is a source of amino acids including tryptophan. Tryptophan is an amino acid used by the body to synthesize serotonin and melatonin neurotransmitters necessary for regulating sleep cycles and moods. Tryptophan amino acid helps easing insomnia, anxiety disorder, and also for mood enhancement, among others beneficial effects to health. It has been suggested that eating pumpkin seeds along with carbohydrates can help achieve a restful sleep.9-12


Antioxidant properties of pumpkin seed (Cucurbita pepo) protein.


An in vivo study investigating pumpkin seed (Cucurbita pepo) protein isolate antioxidant properties was carried out in a carbon tetrachloride-induced acute liver injury in low-protein fed Sprague-Dawley rat model. The study shown the potential components of the pumpkin seed protein isolate with antiperoxidative properties and their effectiveness in alleviating the detrimental effects associated with protein malnutrition and CCl4 intoxication.13 Another study also showed the antioxidative properties of pumpkin seed (Cucurbita pepo) protein isolate and its capacity alleviating the detrimental effects associated with protein malnutrition.14


Anthelmintic activity.


Traditionally, pumpkin seeds have been used to immobilize and to aid the expulsion of intestinal worms and parasites. Also, some cultures eat pumpkin seeds to prevent worm infections. The anthelmintic effectiveness of dried seeds and extracts has been demonstrated in several in vitro and in vivo studies.15-18 Studies carried out from Chinese traditional medicine have showed the antichistosomal effect of cucurbitin, an amino acid isolated from pumpkin seeds, against S. japonicum.19


 Effect on bladder-stone disease


The effect of pumpkin-seed supplementation on oxalcrystalluria and urinary compositions was studied on children in hyperendemic area. Pumpkin seeds lowered calcium-oxalate crystal occurrence and calcium level but increased phosphorus, pyrophosphate, glycosaminoglycans, and potassium values in urine. This clinical trial showed that pumpkin seeds can be used as a potential agent in lowering the risk of bladder-stone disease.20


Protective effect on Benign prostatic hyperplasia (BPH)

Recent studies have shown that pumpkin seed can be useful for the treatment of benign prostate hyperplasia, because of its high β-sitosterol content. Also, β-Sitosterol has been indicated to reduce blood cholesterol and to decrease risks of certain types of cancers.Furthermore, other studies have reported that pumpkin seed may have antiandrogenic and anti-inflammatory activity to help easing benign prostatic hyperplasia (BPH). Clinical trials showed that pumpkin seeds preparations improved symptoms associated with BPH such as urinary frequency, urinary flow, residual urine, and micturition time versus placebo.21-24


Cardiovascular effects


An in vivo study with the objective to examine the effect of pumpkin (Cucurbita pepo L.) seeds supplementation on atherogenic diet-induced atherosclerosis showed that treatment of atherogenic rats with pumpkin seeds significantly decreased serum concentrations of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C).25


Antidiabetic effects


The hypoglycemic and antioxidant effects of flax and pumpkin seeds mixture was investigated on the kidney of diabetic rats. Diet supplemented with this mixture of seeds ameliorated the antioxidant enzymes activities in diabetic rats, suggesting that flax and pumpkin seeds mixture supplemented in diet of diabetic rats may be helpful to prevent diabetes and its complications.26


Food applications


Pumpkin Seed Protein Powder 45 % is a Super Food with wide potential for the elaboration of protein bars, baking, desserts, baked, fortified breads, cakes, cookies, brownies, muffins, pancakes, protein crepes, protein waffles, plant protein burgers, high-protein pastas, snacks, etc. It can also be consumed in smoothies, shakes, beverages, juices, breakfast cereals and when required to fortify foodstuff with proteins of the highest quality nutritional value.


The interest for protein pumpkin seeds has led to current research on its health benefits. A study was carried out to evaluate the addition of pumpkin protein concentrate and pumpkin protein isolate into wheat flour to produce blends with several protein levels. Results indicated that pumpkin seed products and protein isolate can be added to wheat flour in certain proportion without a detrimental effect on dough or loaf quality for bread fortification. Moreover, the addition of pumpkin seed proteins resulted in increasing protein, amino acids and mineral contents compared to the control.27


The functional properties, antioxidant activity, and dietary fiber content of pumpkin seeds and rind was studied in bread formulations substituted with several contents of pumpkin seed and rind. Results showing high content of fiber, scavenging activity, and overall acceptability and sensory characteristics allow the application of pumpkin seeds and rind as healthy bakery product ingredients.28


Other study carried out to evaluated the effect of incorporation of fiber rich pumpkin powder and guar gum on the farinographic characteristics of wheat flour, concluded that pumpkin seedpowder that can be utilized with guar gum forfortifying breads and baked.29 


Food allergy to pumpkin seed and toxicity


Some cases of Cucurbitaceae family allergy has been reported. Oral allergy syndrome pruritus, diarrhea and nausea have occurred in a number of patients. Also, cross-reactivity to watermelon, cucumber, and pumpkin has been described.30-32 Unlike the other members of the Cucurbitaceae family, pumpkin seed is not a common cause of food allergy, and there have been few reports of allergic reactions after ingestion.33,34


Adverse reactions and severe toxicity have not been reported with the use of Cucurbita extracts. Studies of C. maxima ingestion indicate that seeds of C. max are not toxic for rats and swine.35


Organic Pumpkin Seed Protein Powder 45% of The Green Labs


Organic Pumpkin Seed Protein (Cucurbita pepo L.) Powder of The Green Labs is a plant high protein food, rich in vitamins, minerals, and anti-oxidants. It has a wide variety of applications for the development of nutraceuticals, dietary supplements, and for preparation of food and beverages. Organic Pumpkin Seed Protein (Cucurbita pepo L.) Powder of The Green Labs is an organic raw material, non-genetically modified (Non-GMO) and gluten-free.

1.    Available in http://www.kew.org/science-conservation/plants-fungi/cucurbita-pepo-pumpkin

2.    Cucurbita pepo L. The PLANTS Database http://plants.usda.gov/core/profile?symbol=CUPEP

3.    Available in https://hort.purdue.edu/newcrop/1492/cucurbits.html.”Cucurbits”. Purdue University.

4.    Mi Young Kim et al. Comparison of the chemical compositions and nutritive values of various pumpkin

5.    (Cucurbitaceae) species and parts. Nutr Res Pract. 2012 Feb; 6(1): 21–27.

6.    Sicilia T et al. Identification and stereochemical characterization of lignans in flaxseed and pumpkin seeds. J Agric Food Chem. 2003 Feb 26;51(5):1181-8.

7.    Zhang, B. et al (March 2012). Cucurmosin induces apoptosis of BxPC-3 human pancreatic cancer cells via inactivation of the EGFR signaling pathway. Oncology Reports.2011. 27 (3): 891–897.

8.    Hou, Xiaomin et al. Atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of Cucurbita moschata. Journal of Structural Biology. 2008164 (1): 81–87.

9.    Jieming, Xie et al. Antitumor effects of cucurmosin in human chronic myeloid leukemia occur through cell cycle arrest and decrease the Bcl-2/Bax ratio to induce apoptosis. African Journal of Pharmacy and Pharmacology. 2011. 5 (7): 985–992.

10.    Kim MY et al. Comparison of the chemical compositions and nutritive values of various pumpkin (Cucurbitaceae) species and parts. Nutr Res Pract. 2012 Feb;6(1):21-7

11.    Zdunczyk Z et al. Comparative study of the chemical composition and nutritional value of pumpkin seed cake, soybean meal and casein. Nahrung. 1999 Dec;43(6):392-5.

12.    Hudson C, Hudson S, MacKenzie J. Protein-source tryptophan as an efficacious treatment for social anxiety disorder: a pilot study. Can J PhysiolPharmacol . 2007;85(9):928-932.

13.    Hudson C, Hudson SP, Hecht T, MacKenzie J. Protein source tryptophan versus pharmaceutical grade tryptophan as an efficacious treatment for chronic insomnia. NutrNeurosci . 2005;8(2):121-127.

14.    Nkosi CZ et al. Antioxidative effects of pumpkin seed (Cucurbita pepo) protein isolate in CCl4-induced liver injury in low-protein fed rats. Phytother Res. 2006 Nov;20(11):935-40.

15.    Nkosi CZ et al. In Vitro antioxidative activity of pumpkin seed (Cucurbita pepo) protein isolate and its In Vivo effect on alanine transaminase and aspartate transaminase in acetaminophen-induced liver injury in low protein fed rats. Phytother Res. 2006 Sep;20(9):780-3.

16.    De Amorim A, et al. Anthelmintic action of plants. Part 6. Influence of pumpkin seeds in the removal of Vampirolepis nana from mice. Rev Bras Farmacogn. 1992;73:81-82.

17.    Elisha E, et al. Anthelmintic activity of some Iraqi plants of the Cucurbitaceae. Pharm Biol. 1987;25(3):153-157.

18.    Amorim CZ, et al. Screening of the antimalarial activity of plants of the Cucurbitaceae family. Mem Inst Oswaldo Cruz .1991;86(suppl 2):177-180.

19.    Feitosa TF et al. Anthelmintic efficacy of pumpkin seed (Cucurbita pepo Linnaeus, 1753) on ostrich gastrointestinal nematodes in a semiarid region of Paraíba State, Brazil. Trop Anim Health Prod. 2013 Jan;45(1):123-7

20.    Xiao SH et al. Research and development of antischistosomal drugs in the People’s Republic of China a 60-year review. AdvParasitol. 2010;73:231-95

21.    Suphakarn VS et al. The effect of pumpkin seeds on oxalcrystalluria and urinary compositions of children in hyperendemic area. Am J ClinNutr. 1987 Jan;45(1):115-21.

22.    Vahlensieck W et al. Effects of pumpkin seed in men with lower urinary tract symptoms due to benign prostatic hyperplasia in the one-year, randomized, placebo-controlled GRANU study. Urol Int. 2015;94(3):286-95

23.    Dreikorn K. The role of phytotherapy in treating lower urinary tract symptoms and benign prostatic hyperplasia. World J Urol . 2002;19(6):426-435

24.    Gossell-Williams M, Davis A, O’Connor N. Inhibition of testosterone-induced hyperplasia of the prostate of sprague-dawley rats by pumpkin seed oil. J Med Food . 2006;9(2):284-286

25.    Carbin B, Larsson B, Lindahl O. Treatment of benign prostatic hyperplasia with phytosterols. Br J Urol . 1990;66(6):639-641.

26.    Abuelgassim AO, Al-showayman SI. The effect of pumpkin (Cucurbita pepo L) seeds and L-arginine supplementation on serum lipid concentrations in atherogenic rats. Afr J Tradit Complement Altern. 2011 Oct 2;9(1):131-7.

27.    Makni M et al. Flax and Pumpkin seeds mixture ameliorates diabetic nephropathy in rats. Food ChemToxicol. 2010 Aug-Sep;48(8-9):2407-12.

28.    El-Soukkary FA. Evaluation of pumpkin seed products for bread fortification. Plant Foods Hum Nutr. 2001;56(4):365-84.

29.    Nyam KL, Lau M, Tan CP. Fibre from pumpkin (Cucurbita pepo L.) seeds and rinds: physico-chemical properties, antioxidant capacity and application as bakery product ingredients. Malays J Nutr. 2013 Apr;19(1):99-109.

30.    Kundu H et al. Effect of incorporation of pumpkin (Cucurbita moshchata) powder and guar gum on the rheological properties of wheat flour. J Food Sci Technol. 2014 Oct;51(10):2600-7.

31.    Reindl J, Anliker MD, Karamloo F, Vieths S, Wüthrich B. Allergy caused by ingestion of zucchini ( Cucurbita pepo ): characterization of allergens and cross-reactivity to pollen and other foods. J AllergyClinImmunol. 2000;106(2):379-385.

32.    Figueredo E, Cuesta-Herranz J, Minguez A, et al. Allergy to pumpkin and cross-reactivity to other Cucurbitaceae fruits. J Allergy ClinImmunol. 2000;106(2):402-403.

33.    Potter T, Hashimoto K. Butternut squash (Cucurbita moschata) dermatitis. Contact Dermatitis. 1994;30(2):123.

34.    Fritsch R, Ebner H, Kraft D, Ebner C. Food allergy to pumpkinseed-characterization of allergens. Allergy. 1997;52:335-7.

35.    Rodríguez-Jiménez B et al. Food allergy to pumpkin seed. Allergologia et Immunopathologia. Vol. 38. Núm. 01. Enero 2010 – Febrero 2010.

36.    de Queiroz-Neto A, Mataqueiro MI, Santana AE, Alessi AC. Toxicologic evaluation of acute and subacute oral administration of Cucurbita maxima seed extracts to rats and swine.JEthnopharmacol. 1994;43(1):45-51.


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